Simple hanging drop (20 microliter) double antibody radioimmunoassay of human IgM, IgG, IgA, IgD and IgE.

Abstract

A simple hanging drop radioimmunoassay has been developed for different classes of human immunoglobulin from serum and culture supernatant samples. The immune precipitate was formed in inverted Terasaki plates (20 microliter per well) using 4 aliquots (5 microliter) as follows: (A) a standard or unknown sample; (B) rabbit antisera to the appropriate… (More)

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