Simple enzymatic method for determining 1,5-anhydro-D-glucitol in plasma for diagnosis of diabetes mellitus.

@article{Yabuuchi1989SimpleEM,
  title={Simple enzymatic method for determining 1,5-anhydro-D-glucitol in plasma for diagnosis of diabetes mellitus.},
  author={Masahiko Yabuuchi and Mitsutoshi Masuda and Kenji Katoh and Toshio Nakamura and Hiroshi Akanuma},
  journal={Clinical chemistry},
  year={1989},
  volume={35 10},
  pages={2039-43}
}
We have developed a simple method for determination of 1,5-anhydro-D-glucitol in plasma, based on use of pyranose oxidase (EC 1.1.3.10), an enzyme with specificity toward pyranoid compounds such as 1,5-anhydro-D-glucitol and glucose. Plasma samples deproteinized with trichloroacetic acid are passed through a two-layer mini-column packed with strongly basic anion (OH- form, the upper layer) and strongly acidic cation (H+ form, the lower layer) exchange resins. 1,5-Anhydro-D-glucitol is… CONTINUE READING
14 Extracted Citations
14 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 14 extracted citations

Referenced Papers

Publications referenced by this paper.
Showing 1-10 of 14 references

Validity of serum 1,5-anhydroglucitol measurements in diabetes mellitus

  • T Yamanouchi, K Kosaka, T Toyota
  • J Jpn Diabetes Soc
  • 1989

Reduction and recovery of plasma i,5-anhydroD-glucitol level in diabetes mellitus

  • T Yamanouchi, H Akanuma, T Asano, C Komshi, I Akaoka, Y. Akanuma
  • Diabetes
  • 1987
13 Excerpts

mellitus

  • T Nakamura, A Naito, Y Takahashi, H Akanuma
  • 1987

Purification and properties of pyrenose oxidase from Coriolus versicolor

  • Y Macbids, T. Nakanishi
  • Agr Biol Chem
  • 1984
1 Excerpt

Similar Papers

Loading similar papers…