Simple determination of 3alpha-hydroxy fraction and 3beta-hydroxy fraction of urinary 17-ketosteroids.

@article{Yamaguchi1976SimpleDO,
  title={Simple determination of 3alpha-hydroxy fraction and 3beta-hydroxy fraction of urinary 17-ketosteroids.},
  author={Yoshihisa Yamaguchi and C. T. Hayashi and Yuichi Kumahara},
  journal={Clinical chemistry},
  year={1976},
  volume={22 7},
  pages={1066-9}
}
We describe a method for determination of the 3alpha-hydroxysteroid fraction and 3beta-hydroxysteroid fraction of 17-ketosteroids in urine. The method includes enzymatic hydrolysis with beta-glucuronidase and extraction with ethyl acetate and color development of 3beta-hydroxysteroids by 3beta-hydroxysteroid oxidase and of total 17-ketosteroids by the… CONTINUE READING