Simple computer-aided approach for the analyses of the nuclear-magnetic-resonance spectra of histones. Fractions F1, Fsa1, F2B, cleaved halves of F2B and F2B-DNA.

@article{Bradbury1972SimpleCA,
  title={Simple computer-aided approach for the analyses of the nuclear-magnetic-resonance spectra of histones. Fractions F1, Fsa1, F2B, cleaved halves of F2B and F2B-DNA.},
  author={E. Bradbury and H. Rattle},
  journal={European journal of biochemistry},
  year={1972},
  volume={27 2},
  pages={
          270-81
        }
}
A simple method for the computer simulation of high-resolution nuclear magnetic resonance spectra is described and applied to the analyses of salt-induced changes in the spectra of histones. The method is more objective than the earlier approach of visual examination of spectral changes. It allows the information contained in the spectral envelope to be used and has led to some modifications of the earlier proposals of the segments of histones involved in salt-induced conformational changes… Expand
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References

SHOWING 1-10 OF 25 REFERENCES
Proton magnetic resonance studies of conformational changes n cleaved halves of histone F2B.
TLDR
The whole molecule has been examined and the possibility of the formation of some secondary structure in the basic region of the molecule cannot be excluded and evidence in support of this scheme has been obtained. Expand
An investigation of the conformational changes in histones F1 and F2a1 by proton magnetic resonance spectroscopy.
TLDR
High resolution nuclear magnetic resonance spectroscopy is used to study conformational changes in histone fractions F2a1 and F1 when the ionic strength of aqueous solutions is raised and the proportion of amino acid residues incorporated into secondary structure is less than that involved in the conformationalChanges indicated by thenuclear magnetic resonance results. Expand
An investigation of the conformational changes of histone F2b by high resolution nuclear magnetic resonance.
The recent sequence determination of histone F2b has allowed a detailed nuclear magnetic resonance study of the conformational changes and interactions of this protein with (a) increasing ionicExpand
Proton magnetic resonance ttudies of the interactions of histones F1 and F2B with DNA.
TLDR
The characterization of two different regions led to the suggestion that the basic regions of the polypeptide chains are the primary sites of interaction with DNA, while the non-basic regions have the potential for the formation of definite conformations and might be involved in specific interactions other than with DNA. Expand
Conformational Investigations of Histones
WE have carried out some investigations into the conformations of whole histones and histone fractions under various conditions. The experiments on repression of DNA-dependent RNA synthesis withExpand
The conformational changes of calf-thymus histone fractions as determined by the optical rotary dispersion.
TLDR
The optical rotatory dispeersion data indicate that no appreciable denaturation takes place during the fractionation of histones. Expand
Nuclear-magnetic resonance and optical-spectroscopic studies of conformation and interactions in the cleaved halves of histone F2B.
In a previous study it was proposed that salt-induced intrachain and interchain interactions in histone F2B were located largely in the carboxyl half of the molecule. To confirm this proposal F2B wasExpand
Spectroscopic studies of the conformations of histones and protamine
Abstract Earlier optical rotatory dispersion studies on calf thymus histone fractions showed that increasing the salt molarity of aqueous solution caused an increase in the optical rotatoryExpand
Amino-acid Sequence of Slightly Lysine-rich Histone
TLDR
A new chromatographic method using carboxymethyl-cellulose with a linearly decreasing concentration of ethanol in 2.6 M formic acid containing 0.1 M sodium formate as an eluting agent for fractionation and a new polyacrylamide gel electrophoretic system for the characterization of the histone fractions are developed. Expand
A method for the selective extraction of histone fractions f2(a)1 and f2(a)2 from calf thymus deoxyribonucleoprotein at pH7.
  • E. W. Johns
  • Chemistry, Medicine
  • The Biochemical journal
  • 1967
TLDR
Modifications of existing electrophoretic methods are described that enable these fractions to be more easily characterized. Expand
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