Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis.

@article{You2012SimpleCV,
  title={Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis.},
  author={Chun Xue You and Xiao-zhou Zhang and Yi-Heng Percival Zhang},
  journal={Applied and environmental microbiology},
  year={2012},
  volume={78 5},
  pages={1593-5}
}
We developed a general restriction enzyme-free and ligase-free method for subcloning up to three DNA fragments into any location of a plasmid. The DNA multimer generated by prolonged overlap extension PCR was directly transformed in Escherichia coli [e.g., TOP10, DH5α, JM109, and BL21(DE3)] and Bacillus subtilis for obtaining chimeric plasmids. 

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