Simple calibration of TIR field depth using the supercoiling response of DNA.

Abstract

The combination of single-molecule fluorescence and nanomanipulation techniques into a single experimental platform enables one to carry out correlative analysis of the composition and the activity of complex, multicomponent molecular systems. Here we describe implementation and calibration of such a combined system allowing simultaneous single-molecule… (More)
DOI: 10.1016/j.ymeth.2016.03.028

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