Simple approach to reducing proteolysis during secretory production of human parathyroid hormone in Saccharomyces cerevisiae.

@article{Chung1998SimpleAT,
  title={Simple approach to reducing proteolysis during secretory production of human parathyroid hormone in Saccharomyces cerevisiae.},
  author={Bong Hyun Chung and Kwang Sung Park},
  journal={Biotechnology and bioengineering},
  year={1998},
  volume={57 2},
  pages={245-9}
}
A gene coding for human parathyroid hormone (hPTH) was synthesized and cloned into a yeast expression and secretion vector containing the mating factor alpha pre-pro leader sequence and the galactose-inducible promoter, GAL10. The intact hPTH(1-84) was found to be secreted into the culture medium. As observed in the previous reports on the secretory production of hPTH in yeast, however, the proteolytic cleavage occurred as the culture proceeded, resulting in a significant loss of the intact… CONTINUE READING

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