Simple and reliable method to precipitate proteins from bacterial culture supernatant.

Abstract

A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional… (More)

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Cite this paper

@article{Caldwell2004SimpleAR, title={Simple and reliable method to precipitate proteins from bacterial culture supernatant.}, author={Randolph B. Caldwell and Claus T Lattemann}, journal={Applied and environmental microbiology}, year={2004}, volume={70 1}, pages={610-2} }