Simple and rapid preparation of plasmid template by a filtration method using microtiter filter plates.

@article{Itoh1997SimpleAR,
  title={Simple and rapid preparation of plasmid template by a filtration method using microtiter filter plates.},
  author={Masayoshi Itoh and Piero Carninci and Sumiharu Nagaoka and Naokazu Sasaki and Yasushi Okazaki and Tomoya Ohsumi and Masami Muramatsu and Yoshihide Hayashizaki},
  journal={Nucleic acids research},
  year={1997},
  volume={25 6},
  pages={1315-6}
}
We developed a new simple high-throughput plasmid DNA extraction procedure, based on a modified alkaline lysis method, using only one 96-well microtiter glassfilter plate. In this method, cell harvesting, lysis by alkaline and plasmid purification are performed on only one microtiter glassfilter plate. After washing out RNAs or other contaminants, plasmid DNA is eluted by low-ion strength solution, although precipitated chromosomal DNA is not eluted. The plasmid prepared by this method can be… CONTINUE READING