Simple and rapid detection of the porcine reproductive and respiratory syndrome virus from pig whole blood using filter paper.

@article{Inoue2007SimpleAR,
  title={Simple and rapid detection of the porcine reproductive and respiratory syndrome virus from pig whole blood using filter paper.},
  author={Ryo Inoue and Takamitsu Tsukahara and Chinatsu Sunaba and Mitsugi Itoh and Kazunari Ushida},
  journal={Journal of virological methods},
  year={2007},
  volume={141 1},
  pages={102-6}
}
The combination of Flinders Technology Associates filter papers (FTA cards) and real-time PCR was examined to establish a simple and rapid technique for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) from whole pig blood. A modified live PRRS vaccine was diluted with either sterilised saline or pig whole blood, and the suspensions were applied onto the FTA cards. The real-time RT-PCR detection of PRRSV was performed directly with the samples applied to the FTA card… CONTINUE READING
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