Simple and rapid detection of factor V Leiden by allele-specific PCR amplification.

@article{Blasczyk1996SimpleAR,
  title={Simple and rapid detection of factor V Leiden by allele-specific PCR amplification.},
  author={Rainer Blasczyk and Markus Ritter and Christian Thiede and J Wehling and G{\"u}nter Hintz and Andreas Neubauer and Hanno B Riess},
  journal={Thrombosis and haemostasis},
  year={1996},
  volume={75 5},
  pages={757-9}
}
Resistance to activated protein C is the most common hereditary cause for thrombosis and significantly linked to factor V Leiden. In this study, primers were designed to identify the factor V mutation by allele-specific PCR amplification. 126 patients with thromboembolic events were analysed using this technique, PCR-RFLP and direct sequencing. The concordance between these techniques was 100%. In 27 patients a heterozygous factor VGln506 mutation was detected, whereas one patient with… CONTINUE READING

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