Simple and inexpensive but highly discriminating method for computer-assisted DNA fingerprinting of Pseudomonas aeruginosa.

@article{AlSamarrai2000SimpleAI,
  title={Simple and inexpensive but highly discriminating method for computer-assisted DNA fingerprinting of Pseudomonas aeruginosa.},
  author={Taha H. Al-Samarrai and Ning Zhang and Iain L Lamont and Liliane Martin and Julia Anna Kolbe and Margaret L. Wilsher and A. S. Morris and Jan Schmid},
  journal={Journal of clinical microbiology},
  year={2000},
  volume={38 12},
  pages={4445-52}
}
We describe here a method for computer-assisted fingerprinting of Pseudomonas aeruginosa. In this method, DNA is digested with SalI, and bands with molecular sizes of >/=9.7 kb are visually scored after electrophoresis on agarose gels. Pattern scores are entered into a Microsoft Excel database. In scoring, the number of bands within each of a set of molecular size ranges is scored, rather than the absolute molecular size of each band, substantially enhancing the speed and reproducibility of the… CONTINUE READING

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