Simple and efficient vectors for retrofitting BACs and PACs with mammalian neoR and EGFP marker genes.

@article{Kaname2001SimpleAE,
  title={Simple and efficient vectors for retrofitting BACs and PACs with mammalian neoR and EGFP marker genes.},
  author={Tadashi Kaname and Clare Huxley},
  journal={Gene},
  year={2001},
  volume={266 1-2},
  pages={147-53}
}
Bacterial artificial chromosomes (BACs) and P1 artificial chromosomes (PACs) are widely used to investigate the functions of genes and genomes in mammalian cells in vitro and in vivo. We have developed a series of vectors which can simply and efficiently be retrofitted onto BACs or PACs. These vectors carry a neoR gene for selection in cells in tissue culture, including ES cells, and also an EGFP gene driven by the strong CAG promoter for quick detection of the DNA in cells. All the plasmids… CONTINUE READING
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