Simple and efficient identification of chromatin modifying complexes and characterization of complex composition.

@article{Lee2013SimpleAE,
  title={Simple and efficient identification of chromatin modifying complexes and characterization of complex composition.},
  author={Jeong-Heon Lee and David G. Skalnik},
  journal={Methods in molecular biology},
  year={2013},
  volume={977},
  pages={
          289-98
        }
}
Affinity purification and mass spectrometry analysis have been used to identify and characterize protein complexes. Wdr82-associated chromatin modifying complexes were purified by single-step FLAG affinity purification from human cells induced to express FLAG-tagged Wdr82. Purified proteins were analyzed by SDS-PAGE and specific protein bands were identified by mass spectrometry. Subsequently, purified proteins were fractionated on sucrose gradient equilibrium centrifugation to determine… CONTINUE READING
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