Simple and effective technical procedures for the packing and application of nanospray PicoFrit HPLC columns for proteomic analyses.

Abstract

A simple procedure was developed for packing PicoFrit HPLC columns with chromatographic stationary phase using a reservoir fabricated from standard laboratory HPLC fittings. Packed columns were mounted onto a stainless steel ultra-low volume precolumn filter assembly containing a 0.5-microm pore size steel frit. This format provided a conduit for the… (More)

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