Simple and Robust Analysis of Cefuroxime in Human Plasma by LC-MS/MS: Application to a Bioequivalence Study

  title={Simple and Robust Analysis of Cefuroxime in Human Plasma by LC-MS/MS: Application to a Bioequivalence Study},
  author={Xingjiang Hu and Mingzhu Huang and J. Liu and Jun-chun Chen and J. Shentu},
  journal={Advances in Pharmacological Sciences},
A simple, robust LC-MS/MS assay for quantifying cefuroxime in human plasma was developed. [...] Key Method Cefuroxime and tazobactam, as internal standard (IS), were extracted from human plasma by methanol to precipitate protein. Separation was achieved on a Zorbax SB-Aq (4.6 × 250 mm, 5  μ m) column under isocratic conditions. The calibration curve was linear in the concentration range of 0.0525-21.0  μ g/mL (r = 0.9998). The accuracy was higher than 90.92%, while the intra- and interday precision were less…Expand

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