Simple, rapid, and simultaneous assay of multiple carboxyl containing phytohormones in wounded tomatoes by UPLC-MS/MS using single SPE purification and isotope dilution.

@article{Fu2012SimpleRA,
  title={Simple, rapid, and simultaneous assay of multiple carboxyl containing phytohormones in wounded tomatoes by UPLC-MS/MS using single SPE purification and isotope dilution.},
  author={Jihong Fu and Jinfang Chu and Xiaohong Sun and Jide Wang and Cunyu Yan},
  journal={Analytical sciences : the international journal of the Japan Society for Analytical Chemistry},
  year={2012},
  volume={28 11},
  pages={1081-7}
}
An efficient simplified isotope dilution method was developed to determine four carboxyl containing phytohormones simultaneously in 200 mg of fresh tomato tissues using ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) with negative electrospray ionization. The four phytohormones are indole-3-acetic acid (IAA), abscisic acid (ABA), jasmonic acid (JA), and salicylic acid (SA). Only one purification step of Oasis MAX solid phase extraction (SPE) was… CONTINUE READING

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