Simple, Rapid and Cost-Effective Method for High Quality Nucleic Acids Extraction from Different Strains of Botryococcus braunii

@inproceedings{Kim2012SimpleRA,
  title={Simple, Rapid and Cost-Effective Method for High Quality Nucleic Acids Extraction from Different Strains of Botryococcus braunii},
  author={Byung-Hyuk Kim and Rishiram Ramanan and Dae-Hyun Cho and Gang-Guk Choi and H Chandra Chand La and Chi-Yong Ahn and Hee-Mock Oh and Hee-Sik Kim},
  booktitle={PloS one},
  year={2012}
}
This study deals with an effective nucleic acids extraction method from various strains of Botryococcus braunii which possesses an extensive extracellular matrix. A method combining freeze/thaw and bead-beating with heterogeneous diameter of silica/zirconia beads was optimized to isolate DNA and RNA from microalgae, especially from B. braunii. Eukaryotic Microalgal Nucleic Acids Extraction (EMNE) method developed in this study showed at least 300 times higher DNA yield in all strains of B… CONTINUE READING

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Primer sets for detecting microalgae belonging to Chlorophyta

  • Kim H-S, Cho D-H, Jo B-H, Kim B-H, Ahn C-Y
  • 2011
1 Excerpt

Comparison of several methods for effective lipid extraction from microalgae

  • Lee J-Y, C Yoo, Jun S-Y, Ahn C-Y, Oh H-M
  • Bioresour Technol
  • 2010
1 Excerpt

Simple method for RNA preparation from cyanobacteria

  • Kim B-H, Oh H-M, Lee Y-K, Choi G-G, Ahn C-Y
  • J Phycol
  • 2006
2 Excerpts

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