Simplagrin, a Platelet Aggregation Inhibitor from Simulium nigrimanum Salivary Glands Specifically Binds to the Von Willebrand Factor Receptor in Collagen and Inhibits Carotid Thrombus Formation In Vivo

@article{Chagas2014SimplagrinAP,
  title={Simplagrin, a Platelet Aggregation Inhibitor from Simulium nigrimanum Salivary Glands Specifically Binds to the Von Willebrand Factor Receptor in Collagen and Inhibits Carotid Thrombus Formation In Vivo},
  author={Andrezza Campos Chagas and Peter McPhie and Hong San and David L. Narum and Karine Reiter and Fuyuki Tokomasu and F{\'a}bio Andr{\'e} Brayner and Luiz Carlos Alves and Jos{\'e} M. C. Ribeiro and Eric Calvo},
  journal={PLoS Neglected Tropical Diseases},
  year={2014},
  volume={8}
}
Background Among the several challenges faced by bloodsucking arthropods, the vertebrate hemostatic response against blood loss represents an important barrier to efficient blood feeding. Here we report the first inhibitor of collagen-induced platelet aggregation derived from the salivary glands of a black fly (Simulium nigrimanum), named Simplagrin. Methods and Findings Simplagrin was expressed in mammalian cells and purified by affinity-and size-exclusion chromatography. Light-scattering… 

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