Simian virus 40 integration sites in the genome of virus-transformed mouse cells

@article{Prasad1975SimianV4,
  title={Simian virus 40 integration sites in the genome of virus-transformed mouse cells},
  author={I. Prasad and D. Zouzias and C. Basilico},
  journal={Journal of Virology},
  year={1975},
  volume={16},
  pages={897 - 904}
}
To gain information on the specificity of simian virus 40 (SV40) integration in the genome of transformed cells, mouse 3T3 cells were transformed by a temperature-sensitive (ts) SV40 mutant, using high multiplicity of infection (MOI). Transformed cells were superinfected with wild-type (wt) virus at high MOI. Clones were isolated and fused with permissive BSC-1 cells to promote virus rescue. All rescued viruses were of the ts type only. When the high-MOI transformants were infected with 3H… Expand
13 Citations
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The transformation of cell growth and transmogrification of DNA synthesis by simian virus 40.
  • R. Martin
  • Biology, Medicine
  • Advances in cancer research
  • 1981
TLDR
The hypothesis is that transformation by SV40 unlike PyV is largely explicable in terms of the ability of the 90K T-antigen to initiate DNA synthesis, which can propel the cell into rounds of replication beyond those exhibited by non-transformed cells in the same conditions. Expand
Symmetric transcription of simian virus 40 DNA in the nuclei of transformed mouse cells.
TLDR
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SV40-transformed human diploid cells that remain transformed throughout their limited lifespan.
Of ninety-one subcultured foci of SV40-transformed WI-38 human diploid fibroblasts, none yielded cells that grew indefinitely, but all cells in each subculture continued to produce T antigen and toExpand
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TLDR
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RECOVERY OF PARAMYXOVIRUS FROM THE JEJUNUM OF PATIENTS WITH MULTIPLE SCLEROSIS
Paramyxovirus, presumably measles, has been recovered by cocultivation or cell-fusion techniques from jejunal biopsy specimens of 6 consecutive patients with multiple sclerosis. Virus has beenExpand
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