Silver staining of proteins in 2DE gels.

  title={Silver staining of proteins in 2DE gels.},
  author={C{\'e}cile Lelong and Mireille Chevallet and Sylvie Luche and Thierry Rabilloud},
  journal={Methods in molecular biology},
Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation, and finally image development. Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 day after the… 
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