Silver Staining of 2D Electrophoresis Gels

@article{Rabilloud2012SilverSO,
  title={Silver Staining of 2D Electrophoresis Gels},
  author={Thierry Rabilloud},
  journal={Methods in molecular biology},
  year={2012},
  volume={893},
  pages={
          61-73
        }
}
  • T. Rabilloud
  • Published 14 June 2012
  • Biology
  • Methods in molecular biology
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It -combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in proteomics, two important additional features must be considered, compatibility with mass spectrometry and quantitative response. Both features are discussed in this chapter, and optimized silver staining protocols are proposed. 
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TLDR
Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 d after the end of the electrophoretic separation, which is compatible with downstream processing, such as mass spectrometry analysis after protein digestion.
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TLDR
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TLDR
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TLDR
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