Silkworm and spider silk scaffolds for chondrocyte support

@article{Gellynck2008SilkwormAS,
  title={Silkworm and spider silk scaffolds for chondrocyte support},
  author={Kris Gellynck and Peter C. M. Verdonk and Els Van Nimmen and Karl Fredrik Almqvist and Tom Gheysens and Gustaaf Schoukens and Lieva Van Langenhove and Paul Kiekens and Johan Mertens and Gust Verbruggen},
  journal={Journal of Materials Science: Materials in Medicine},
  year={2008},
  volume={19},
  pages={3399-3409}
}
Objective To create scaffolds with silkworm cocoon, spider egg sac and spider dragline silk fibres and examine their use for chondrocyte attachment and support. Methods Three different kinds of scaffolds were developed with Bombyx mori cocoon, Araneus diadematus egg sac and dragline silk fibres. The attachment of human articular cartilage cells were investigated on these bioprotein matrices. The chondrocytes produced an extracellular matrix which was studied by immunostaining. Moreover, the… 

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References

SHOWING 1-10 OF 53 REFERENCES

Macrophage responses to silk.

Surface properties of silk fibroin films and their interaction with fibroblasts.

Its non-homogeneous surface can explain the existence of cells spreading in specific directions and the presence of cell repellent areas even 4 d after seeding, while the new treatment proposed could really dialogue with the cells.

Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin.

Synthesis and evaluation of scaffolds prepared from chitosan fibers for potential use in cartilage tissue engineering.

Electrospinning is employed to prepare scaffolds that mimic the native ECM of articular cartilage, and distribute strain in a bioresponsive manner to signal seeded chondrocytes to synthesize and organize ECM to result in material properties that are in range of natural cartilage.

Research and development of spider silk for biomedical applications

In January 2002, a 4-years project started at Ghent University concerning research on spider silk for biomedical applications. This project aims at the development of a textile fabric from spider

Porous 3-D scaffolds from regenerated silk fibroin.

Three fabrication techniques were used to form porous three-dimensional silk biomaterial matrixes and the results suggest that silk-based 3D matrixes can be formed for utility in biomaterial applications.

Tissue engineering of cartilage with the use of chitosan-gelatin complex scaffolds.

  • W. XiaWei Liu Yilin Cao
  • Biology, Engineering
    Journal of biomedical materials research. Part B, Applied biomaterials
  • 2004
It is demonstrated that the chitosan-gelatin complex may serve as a suitable scaffold for cartilage tissue engineering.

Photocrosslinkable Hyaluronan as a Scaffold for Articular Cartilage Repair

Hyaluronan-based scaffolds are of interest for tissue-engineered cartilage repair due to an important role for hyaluronan in cartilage development and function. In this study, an in situ

Characterization of polylactic acid-polyglycolic acid composites for cartilage tissue engineering.

Scanning electron microscopy indicated that cells attached to PGA appeared flat with many small processes, whereas those attached to PLA were more rounded, which provides important information for the design of scaffolds for cartilage tissue engineering.
...