Silkworm and spider silk scaffolds for chondrocyte support

  title={Silkworm and spider silk scaffolds for chondrocyte support},
  author={Kris Gellynck and Peter C. M. Verdonk and Els Van Nimmen and Karl Fredrik Almqvist and Tom Gheysens and Gustaaf Schoukens and Lieva Van Langenhove and Paul Kiekens and Johan Mertens and Gust Verbruggen},
  journal={Journal of Materials Science: Materials in Medicine},
Objective To create scaffolds with silkworm cocoon, spider egg sac and spider dragline silk fibres and examine their use for chondrocyte attachment and support. Methods Three different kinds of scaffolds were developed with Bombyx mori cocoon, Araneus diadematus egg sac and dragline silk fibres. The attachment of human articular cartilage cells were investigated on these bioprotein matrices. The chondrocytes produced an extracellular matrix which was studied by immunostaining. Moreover, the… 

Interactions between Spider Silk and Cells – NIH/3T3 Fibroblasts Seeded on Miniature Weaving Frames

Native spider silk does not require any modification to its application as a biomaterial that can rival any artificial material in terms of cell growth promoting properties, and adhesion mechanics on intracellular level are shown.

Silk fibroin biomaterials for tissue regenerations.

Spider Silk for Tissue Engineering Applications

The research on spider silk-based materials in the field of tissue engineering is highlighted and various two-dimensional and three-dimensional scaffolds made of spider silk are summarized.

Reinforcing silk scaffolds with silk particles.

In this work, porogen leached scaffolds prepared from aqueous and HFIP silk solutions were reinforced through the addition of silk particles, which led to about 40 times increase in the specific compressive modulus and the yield strength of HFIP-based scaffolds.

In Vitro Evaluation of Spider Silk Meshes as a Potential Biomaterial for Bladder Reconstruction

It is demonstrated that spider silk without any additional biological coating supports adhesion and growth of primary human urothelial cells (HUCs), which are multipotent bladder cells able to differentiate into the various epithelial layers of the bladder.

A bilayered scaffold based on RGD recombinant spider silk proteins for small diameter tissue engineering

After all, the intimal surface promotes endothelialization and being highly anti-thrombogenic, while the mismatch in mechanical properties outside induces intimal hyperplasia, it is desirable to develop a bioactive material with improved mechanical properties for small-diameter vascular graft.

Spider silk: an excellent biomaterial for medical science and industry

Spider silk is categorized as fibrous protein. Spiders produce several types of silk. Spider silk is important because of its maximum mechanical strength, biocompatibility and biodegradability, pore

Enhanced osteogenic potential of human mesenchymal stem cells on electrospun nanofibrous scaffolds prepared from eri-tasar silk fibroin.

The ET scaffolds promoted greater differentiation of the attached hMSCs as evidenced by higher expression of RunX2, osteocalcin, and CD29/CD44 expression and significantly higher mineralization, as shown by glycosaminoglycan assay, alizarin red staining, and elemental analysis of crystalline composites isolated from the scaffolds.

Recent Innovations in Silk Biomaterials

This article summarizes the current research related to the formation of silk materials with different morphologies, their biocompatibility, and examples of their biomedical applications.



Macrophage responses to silk.

Surface properties of silk fibroin films and their interaction with fibroblasts.

Its non-homogeneous surface can explain the existence of cells spreading in specific directions and the presence of cell repellent areas even 4 d after seeding, while the new treatment proposed could really dialogue with the cells.

Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin.

Synthesis and evaluation of scaffolds prepared from chitosan fibers for potential use in cartilage tissue engineering.

Electrospinning is employed to prepare scaffolds that mimic the native ECM of articular cartilage, and distribute strain in a bioresponsive manner to signal seeded chondrocytes to synthesize and organize ECM to result in material properties that are in range of natural cartilage.

Research and development of spider silk for biomedical applications

In January 2002, a 4-years project started at Ghent University concerning research on spider silk for biomedical applications. This project aims at the development of a textile fabric from spider

Porous 3-D scaffolds from regenerated silk fibroin.

Three fabrication techniques were used to form porous three-dimensional silk biomaterial matrixes and the results suggest that silk-based 3D matrixes can be formed for utility in biomaterial applications.

Tissue engineering of cartilage with the use of chitosan-gelatin complex scaffolds.

  • W. XiaWei Liu Yilin Cao
  • Biology, Engineering
    Journal of biomedical materials research. Part B, Applied biomaterials
  • 2004
It is demonstrated that the chitosan-gelatin complex may serve as a suitable scaffold for cartilage tissue engineering.

Photocrosslinkable Hyaluronan as a Scaffold for Articular Cartilage Repair

Hyaluronan-based scaffolds are of interest for tissue-engineered cartilage repair due to an important role for hyaluronan in cartilage development and function. In this study, an in situ

Characterization of polylactic acid-polyglycolic acid composites for cartilage tissue engineering.

Scanning electron microscopy indicated that cells attached to PGA appeared flat with many small processes, whereas those attached to PLA were more rounded, which provides important information for the design of scaffolds for cartilage tissue engineering.