Silkworm and spider silk scaffolds for chondrocyte support

@article{Gellynck2008SilkwormAS,
  title={Silkworm and spider silk scaffolds for chondrocyte support},
  author={Kris Gellynck and Peter C. M. Verdonk and Els Van Nimmen and Karl Fredrik Almqvist and Tom Gheysens and Gustaaf Schoukens and Lieva Van Langenhove and Paul Kiekens and Johan Mertens and Gust Verbruggen},
  journal={Journal of Materials Science: Materials in Medicine},
  year={2008},
  volume={19},
  pages={3399-3409}
}
Objective To create scaffolds with silkworm cocoon, spider egg sac and spider dragline silk fibres and examine their use for chondrocyte attachment and support. Methods Three different kinds of scaffolds were developed with Bombyx mori cocoon, Araneus diadematus egg sac and dragline silk fibres. The attachment of human articular cartilage cells were investigated on these bioprotein matrices. The chondrocytes produced an extracellular matrix which was studied by immunostaining. Moreover, the… 
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86 Citations
Highly Influential Citations
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Background Citations
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Methods Citations
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86 Citations

Interactions between Spider Silk and Cells – NIH/3T3 Fibroblasts Seeded on Miniature Weaving Frames

Native spider silk does not require any modification to its application as a biomaterial that can rival any artificial material in terms of cell growth promoting properties, and adhesion mechanics on intracellular level are shown.

Silk fibroin biomaterials for tissue regenerations.

Spider Silk for Tissue Engineering Applications

The research on spider silk-based materials in the field of tissue engineering is highlighted and various two-dimensional and three-dimensional scaffolds made of spider silk are summarized.

Reinforcing silk scaffolds with silk particles.

In this work, porogen leached scaffolds prepared from aqueous and HFIP silk solutions were reinforced through the addition of silk particles, which led to about 40 times increase in the specific compressive modulus and the yield strength of HFIP-based scaffolds.

In Vitro Evaluation of Spider Silk Meshes as a Potential Biomaterial for Bladder Reconstruction

It is demonstrated that spider silk without any additional biological coating supports adhesion and growth of primary human urothelial cells (HUCs), which are multipotent bladder cells able to differentiate into the various epithelial layers of the bladder.

A bilayered scaffold based on RGD recombinant spider silk proteins for small diameter tissue engineering

After all, the intimal surface promotes endothelialization and being highly anti-thrombogenic, while the mismatch in mechanical properties outside induces intimal hyperplasia, it is desirable to develop a bioactive material with improved mechanical properties for small-diameter vascular graft.

Spider silk: an excellent biomaterial for medical science and industry

Spider silk is categorized as fibrous protein. Spiders produce several types of silk. Spider silk is important because of its maximum mechanical strength, biocompatibility and biodegradability, pore…

Enhanced osteogenic potential of human mesenchymal stem cells on electrospun nanofibrous scaffolds prepared from eri-tasar silk fibroin.

The ET scaffolds promoted greater differentiation of the attached hMSCs as evidenced by higher expression of RunX2, osteocalcin, and CD29/CD44 expression and significantly higher mineralization, as shown by glycosaminoglycan assay, alizarin red staining, and elemental analysis of crystalline composites isolated from the scaffolds.

Recent Innovations in Silk Biomaterials

This article summarizes the current research related to the formation of silk materials with different morphologies, their biocompatibility, and examples of their biomedical applications.

A review on advances in the applications of spider silk in biomedical issues.

...

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