Silicon nitride windows for electron microscopy of whole cells.

  title={Silicon nitride windows for electron microscopy of whole cells.},
  author={Elisabeth A Ring and Diana B. Peckys and Madeline J. Dukes and Jean Pierre Baudoin and Niels de Jonge},
  journal={Journal of microscopy},
  volume={243 3},
Silicon microchips with thin, electron transparent silicon nitride windows provide a sample support that accommodates both light-, and electron microscopy of whole eukaryotic cells in vacuum or liquid, with minimum sample preparation steps. The windows are robust enough that cellular samples can be cultured directly onto them, with no addition of a supporting film, and there is no need to embed or section the sample, as is typically required in electron microscopy. By combining two microchips… CONTINUE READING

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