Silica-induced apoptosis in mouse alveolar macrophages is initiated by lysosomal enzyme activity.

  title={Silica-induced apoptosis in mouse alveolar macrophages is initiated by lysosomal enzyme activity.},
  author={Michael Thibodeau and Charles Giardina and David A. Knecht and Joseph J. Helble and Andrea K. Hubbard},
  journal={Toxicological sciences : an official journal of the Society of Toxicology},
  volume={80 1},
Past studies in our laboratory have shown that silica (-quartz) particle exposure of a mouse alveolar macrophage cell line (MH-S) elicits mitochondrial depolarization and caspase 3 and 9 activation, contributing to apoptosis. However, cellular pathways leading to these outcomes have not been extensively investigated. Initial studies revealed that silica exposure elicits lysosomal permeability after 1 h, as evidenced by leakage of FITC-conjugated dextran and acridine orange. We next evaluated a… CONTINUE READING


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