Silencing of genes and alleles by RNAi in Anopheles gambiae.

@article{Lamacchia2013SilencingOG,
  title={Silencing of genes and alleles by RNAi in Anopheles gambiae.},
  author={Marina Lamacchia and John R. Clayton and Rui Wang-Sattler and Lars M. Steinmetz and Elena A. Levashina and St{\'e}phanie A. Blandin},
  journal={Methods in molecular biology},
  year={2013},
  volume={923},
  pages={
          161-76
        }
}
  • Marina Lamacchia, John R. Clayton, +3 authors Stéphanie A. Blandin
  • Published in Methods in molecular biology 2013
  • Biology, Medicine
  • Anopheles gambiae mosquitoes are the major vectors of human malaria parasites. However, mosquitoes are not passive hosts for parasites, actively limiting their development in vivo. Our current understanding of the mosquito antiparasitic response is mostly based on the phenotypic analysis of gene knockdowns obtained by RNA interference (RNAi), through the injection or transfection of long dsRNAs in adult mosquitoes or cultured cells, respectively. Recently, RNAi has been extended to silence… CONTINUE READING

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