Silencing of OPR3 in tomato reveals the role of OPDA in callose deposition during the activation of defense responses against Botrytis cinerea.

@article{Scalschi2015SilencingOO,
  title={Silencing of OPR3 in tomato reveals the role of OPDA in callose deposition during the activation of defense responses against Botrytis cinerea.},
  author={Loredana Scalschi and Maite Sanmart{\'i}n and Gemma Cama{\~n}es and Pilar Troncho and Jos{\'e} Juan S{\'a}nchez-Serrano and Pilar Garc{\'i}a-Agust{\'i}n and Begonya Vicedo},
  journal={The Plant journal : for cell and molecular biology},
  year={2015},
  volume={81 2},
  pages={
          304-15
        }
}
Cis-(+)-12-oxo-phytodienoic acid (OPDA) is likely to play signaling roles in plant defense that do not depend on its further conversion to the phytohormone jasmonic acid. To elucidate the role of OPDA in Solanum lycopersicum (tomato) plant defense, we have silenced the 12-oxophytodienoate reductase 3 (OPR3) gene. Two independent transgenic tomato lines (SiOPR3-1 and SiOPR3-2) showed significantly reduced OPR3 expression upon infection with the necrotrophic pathogen Botrytis cinerea. Moreover… 

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