Significant structural change in both O- and N-linked carbohydrate moieties of the antigenic galactomannan from Aspergillus fumigatus grown under different culture conditions.

  title={Significant structural change in both O- and N-linked carbohydrate moieties of the antigenic galactomannan from Aspergillus fumigatus grown under different culture conditions.},
  author={Atsushi Kudoh and Yoshio Okawa and Nobuyuki Shibata},
  volume={25 1},
Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structural studies of the galactomannan of Aspergillus fumigatus, some inconsistencies are present in these results. In this study, to clarify the relationship between the growth conditions… 

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The 3D structures of the soluble catalytic domain of CmsA/Ktr4, as determined by X-ray crystallography, and a 3D structural model of the enzyme–substrate complex generated using docking and molecular dynamics simulations with α-Man-(1→6)-α-Man-OMe as the model structure for the acceptor substrate are presented.

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An α-(1→6)-mannosyltransferase responsible for the biosynthesis of the α-core-mannan of fungal-type galactomannan is identified, which has not been known for a long time.

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The characterization of the Aspergillus-specific mAb AP3 (IgG1κ) is described, including the precise identification of its corresponding antigen, and it is shown that AP3 captures GM in serum, suggesting it may be useful as a diagnostic tool for patients with IA.



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