Significant structural change in both O- and N-linked carbohydrate moieties of the antigenic galactomannan from Aspergillus fumigatus grown under different culture conditions.

@article{Kudoh2015SignificantSC,
  title={Significant structural change in both O- and N-linked carbohydrate moieties of the antigenic galactomannan from Aspergillus fumigatus grown under different culture conditions.},
  author={Atsushi Kudoh and Yoshio Okawa and Nobuyuki Shibata},
  journal={Glycobiology},
  year={2015},
  volume={25 1},
  pages={
          74-87
        }
}
Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structural studies of the galactomannan of Aspergillus fumigatus, some inconsistencies are present in these results. In this study, to clarify the relationship between the growth conditions… 
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Galactomannan Produced by Aspergillus fumigatus: An Update on the Structure, Biosynthesis and Biological Functions of an Emblematic Fungal Biomarker

Recent studies have revealed the crucial role of the GM during vegetative and polarized fungal growth and its biological functions during the saprophytic and pathogenic life of this opportunistic human fungal pathogen.

Identification of galactofuranose antigens such as galactomannoproteins and fungal-type galactomannan from the yellow koji fungus (Aspergillus oryzae)

Proton nuclear magnetic resonance spectrometry revealed that the galactofuranose-containing sugar chain was deficient and that core mannan backbone structures were present in ΔugmA A. oryzae, indicating the presence of fungal-type galactomannan in the cell wall fraction of A.oryzae.

GfsA is a &bgr;1,5-galactofuranosyltransferase involved in the biosynthesis of the galactofuran side chain of fungal-type galactomannan in Aspergillus fumigatus

An in vitro assay to measure galactofuranosyltransferase activity in Aspergillus fumigatus revealed that AfGfsA is responsible for the biosynthesis of β1,5-galactofuranose in the galact ofuran side chain of fungal-type galactomannan.

[Preparation and Biological Characterization of Limulus Factor G-activating Substance of Aspergillus spp.]

It is demonstrated that the ASWS corresponds to the limulus factor G-activating substance found in the blood of patients with aspergillosis, and was eliminated from the blood more rapidly than Aspergillus solubilized cell wall β-glucan.

Synthesis of biotinylated pentasaccharide structurally related to a fragment of glucomannan from Candida utilis

This paper describes the first synthesis of a pentasaccharide structurally related to C. utilis glucomannan fragment, which is an α-(1→2)-linked tetramannoside terminated at the non-reducing end by an α-d-glucopyranosyl residue.

Structural basis for the core-mannan biosynthesis of cell wall fungal-type galactomannan in Aspergillus fumigatus

The 3D structures of the soluble catalytic domain of CmsA/Ktr4, as determined by X-ray crystallography, and a 3D structural model of the enzyme–substrate complex generated using docking and molecular dynamics simulations with α-Man-(1→6)-α-Man-OMe as the model structure for the acceptor substrate are presented.

Characterization of a PA14 domain-containing galactofuranose-specific β-d-galactofuranosidase from Streptomyces sp.

Describing a strain of Streptomyces strain JHA26, in the culture supernatant of which exhibited Galf-ase activity for 4-nitrophenyl β-d-galactofuranoside (pNP-β- d-Galf) as a substrate, revealed a putative gene, termed ORF0643, that encodes Galf -ase containing a PA14 domain, which is thought to function in substrate recognition.

Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.

Identification of an α-(1→6)-Mannosyltransferase Contributing To Biosynthesis of the Fungal-Type Galactomannan α-Core-Mannan Structure in Aspergillus fumigatus

An α-(1→6)-mannosyltransferase responsible for the biosynthesis of the α-core-mannan of fungal-type galactomannan is identified, which has not been known for a long time.

Monoclonal Antibody AP3 Binds Galactomannan Antigens Displayed by the Pathogens Aspergillus flavus, A. fumigatus, and A. parasiticus

The characterization of the Aspergillus-specific mAb AP3 (IgG1κ) is described, including the precise identification of its corresponding antigen, and it is shown that AP3 captures GM in serum, suggesting it may be useful as a diagnostic tool for patients with IA.

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