• Corpus ID: 201043145

Significant improvement in in vitro development of sheep hand-made cloned embryos using epigenetic modifiers in culture following nuclear tranfer

  title={Significant improvement in in vitro development of sheep hand-made cloned embryos using epigenetic modifiers in culture following nuclear tranfer},
  author={Sajad A. Beigh and Wani Abdul Ahad and Jaan Mohammad Wani and Mir Mehrajudin and Syed Mehmmod and W. A. Malla and Shafqat Majeed Khan and Riaz Ahmad Shah},
  journal={Journal of entomology and zoology studies},
The present study was carried with the use of reprogramming enhancers trichostatin A (TSA), a histone deacetylase inhibitor (HDACi) and 5-aza-2-deoxycytidine (5-aza-dc), a DNA methyl transferase inhibitor on hand-made cloned (HMC) embryos. It was found that TSA treatment (50 nM) and 5-aza-dc (25nM) for 24 hours following oocyte activation, improved in vitro HMC embryo development. Cleavage percentage observed on Day 2, in case of TSA treated HMC embryos was found to be significantly (p<0.05… 

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    Proceedings of the National Academy of Sciences of the United States of America
  • 2001
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