Significant improvement in in vitro development of sheep hand-made cloned embryos using epigenetic modifiers in culture following nuclear tranfer
@article{Beigh2018SignificantII, title={Significant improvement in in vitro development of sheep hand-made cloned embryos using epigenetic modifiers in culture following nuclear tranfer}, author={S. A. Beigh and Wani A. Ahad and Jaan Mohammad Wani and Mir Mehrajudin and Syed Mehmmod and W. A. Malla and Shafqat Majeed Khan and R. Shah}, journal={Journal of entomology and zoology studies}, year={2018}, volume={6}, pages={54-58} }
The present study was carried with the use of reprogramming enhancers trichostatin A (TSA), a histone deacetylase inhibitor (HDACi) and 5-aza-2-deoxycytidine (5-aza-dc), a DNA methyl transferase inhibitor on hand-made cloned (HMC) embryos. It was found that TSA treatment (50 nM) and 5-aza-dc (25nM) for 24 hours following oocyte activation, improved in vitro HMC embryo development. Cleavage percentage observed on Day 2, in case of TSA treated HMC embryos was found to be significantly (p<0.05… CONTINUE READING
References
SHOWING 1-10 OF 29 REFERENCES
Effects of Trichostatin A on In vitro Development of Porcine Embryos Derived from Somatic Cell Nuclear Transfer
- Biology, Medicine
- Asian-Australasian journal of animal sciences
- 2013
- 10
- PDF
Increased pre-implantation development of cloned bovine embryos treated with 5-aza-2'-deoxycytidine and trichostatin A.
- Biology, Medicine
- Theriogenology
- 2008
- 149
Treating Cloned Embryos, But Not Donor Cells, with 5-aza-2’-deoxycytidine Enhances the Developmental Competence of Porcine Cloned Embryos
- Biology, Medicine
- The Journal of reproduction and development
- 2013
- 35
- Highly Influential
- PDF
The effects of 5-aza-2'- deoxycytidine and trichostatin A on gene expression and DNA methylation status in cloned bovine blastocysts.
- Biology, Medicine
- Cellular reprogramming
- 2011
- 69
Effects of 5-aza-2’-deoxyctidine on the development of porcine parthenogenetic and nuclear transfer embryos
- Biology
- 2013
- 2
- PDF
Treatment of buffalo (Bubalus bubalis) donor cells with trichostatin A and 5-aza-2'-deoxycytidine alters their growth characteristics, gene expression and epigenetic status and improves the in vitro developmental competence, quality and epigenetic status of cloned embryos.
- Biology, Medicine
- Reproduction, fertility, and development
- 2016
- 23
Conservation of methylation reprogramming in mammalian development: Aberrant reprogramming in cloned embryos
- Biology, Medicine
- Proceedings of the National Academy of Sciences of the United States of America
- 2001
- 927
- PDF
Pluripotency Associated Genes Are Reactivated by Chromatin‐Modifying Agents in Neurosphere Cells
- Biology, Medicine
- Stem cells
- 2008
- 91