Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system.

@article{Zhang2011SignificantEO,
  title={Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system.},
  author={Zhi-Jun Zhang and Jiang Qiong Pan and J F Liu and Jian-he Xu and Yucai He and You-Yan Liu},
  journal={Journal of biotechnology},
  year={2011},
  volume={152 1-2},
  pages={
          24-9
        }
}
The enantioselective hydrolysis of mandelonitrile with whole cells of a recombinant Escherichia coli expressing nitrilase activity was severely inhibited by the substrate at high concentrations (>300mM), which resulted in a low yield of the target product (R)-(-)-mandelic acid. To relieve the substrate inhibition and to enhance the (R)-(-)-mandelic acid productivity, eight water-organic solvent biphasic systems were attempted in this work. Toluene was found to be the most suitable solvent as… CONTINUE READING
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