Significance of serum factors in the stimulation of human umbilical cord and fetal lymphocytes by soluble protein A from Staphylococcus aureus.

@article{Turunen1979SignificanceOS,
  title={Significance of serum factors in the stimulation of human umbilical cord and fetal lymphocytes by soluble protein A from Staphylococcus aureus.},
  author={Ossi Turunen},
  journal={European journal of immunology},
  year={1979},
  volume={9 8},
  pages={
          597-601
        }
}
Human umbilical cord and fetal lymphocytes were cultured in the presence of various sera [AB serum, fetal calf serum (FCS) and gammaglobulin-free FCS] and soluble protein A from Staphylococcus aureus (SpA). Dose response studies showed that SpA in a final concentration of 100 μg/ml stimulated moderately cord blood lymphocytes. SpA-induced DNA synthesis was significantly higher when cord lymphocytes were cultured with FCS than with AB serum or gammaglobulin-free FCS. After 6 days' culture, even… CONTINUE READING