Signature-tagged mutagenesis: barcoding mutants for genome-wide screens

  title={Signature-tagged mutagenesis: barcoding mutants for genome-wide screens},
  author={Piotr S Mazurkiewicz and Christoph M Tang and Charles Boone and David W. Holden},
  journal={Nature Reviews Genetics},
DNA signature tags (molecular barcodes) facilitate functional screens by identifying mutants in mixed populations that have a reduced or increased adaptation to a particular environment. Many innovative adaptations and refinements in the technology have been described since its original use with Salmonella; they have yielded a wealth of information on a broad range of biological processes — mainly in bacteria, but also in yeast and other fungi, viruses, parasites and, most recently, in… CONTINUE READING