Signaling through transforming G protein-coupled receptors in NIH 3T3 cells involves c-Raf activation. Evidence for a protein kinase C-independent pathway.

@article{Crespo1994SignalingTT,
  title={Signaling through transforming G protein-coupled receptors in NIH 3T3 cells involves c-Raf activation. Evidence for a protein kinase C-independent pathway.},
  author={Piero Crespo and Ningzhi Xu and Jos{\'e} Luis Daniotti and J Troppmair and Ulf R{\"u}diger Rapp and J. Silvio Gutkind},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 33},
  pages={21103-9}
}
We have studied the role of Raf-1 in mitogenesis and cellular transformation induced by G protein-coupled receptors in NIH 3T3 cells transfected with the human m1 muscarinic receptor. We have observed that in m1-expressing NIH 3T3 cells, the cholinergic agonist carbachol induces a dose- and time-dependent shift in the electrophoretic mobility of p72Raf-1, equivalent to that observed when using phorbol esters or platelet-derived growth factor as stimulants. Phosphoamino acid analysis of slower… CONTINUE READING