Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display

@article{Steiner2006SignalSD,
  title={Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display},
  author={Daniel Steiner and Patrik Forrer and Michael T Stumpp and Andreas Pl{\"u}ckthun},
  journal={Nature Biotechnology},
  year={2006},
  volume={24},
  pages={823-831}
}
Even proteins that fold well in bacteria are frequently displayed poorly on filamentous phages. Low protein presentation on phage might be caused by premature cytoplasmic folding, leading to inefficient translocation into the periplasm. As translocation is an intermediate step in phage assembly, we tested the display levels of a range of proteins using different translocation pathways by employing different signal sequences. Directing proteins to the cotranslational signal recognition particle… CONTINUE READING
70 Citations
53 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 70 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 53 references

A Practical Approach (eds

  • M. Russel, H. B. Lowman, Clackson, T. Introduction to phage biology, phage display. in Phage Display
  • Lowman, H.B. & Clackson, T.) 1–26,
  • 2004

Phage Display: A Practical Approach (Oxford

  • T. Clackson, Lowman, H.B
  • 2004

Similar Papers

Loading similar papers…