Signal regulatory protein α regulates the homeostasis of T lymphocytes in the spleen.

Abstract

The molecular basis for formation of lymphoid follicle and its homeostasis in the secondary lymphoid organs remains unclear. Signal regulatory protein α (SIRPα), an Ig superfamily protein that is predominantly expressed in dendritic cells or macrophages, mediates cell-cell signaling by interacting with CD47, another Ig superfamily protein. In this study, we show that the size of the T cell zone as well as the number of CD4(+) T cells were markedly reduced in the spleen of mice bearing a mutant (MT) SIRPα that lacks the cytoplasmic region compared with those of wild-type mice. In addition, the expression of CCL19 and CCL21, as well as of IL-7, which are thought to be important for development or homeostasis of the T cell zone, was markedly decreased in the spleen of SIRPα MT mice. By the use of bone marrow chimera, we found that hematopoietic SIRPα is important for development of the T cell zone as well as the expression of CCL19 and CCL21 in the spleen. The expression of lymphotoxin and its receptor, lymphotoxin β receptor, as well as the in vivo response to lymphotoxin β receptor stimulation were also decreased in the spleen of SIRPα MT mice. CD47-deficient mice also manifested phenotypes similar to SIRPα MT mice. These data suggest that SIRPα as well as its ligand CD47 are thus essential for steady-state homeostasis of T cells in the spleen.

DOI: 10.4049/jimmunol.1100528

3 Figures and Tables

0100200201220132014201520162017
Citations per Year

200 Citations

Semantic Scholar estimates that this publication has 200 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{SatoHashimoto2011SignalRP, title={Signal regulatory protein α regulates the homeostasis of T lymphocytes in the spleen.}, author={Miho Sato-Hashimoto and Yasuyuki Saito and Hiroshi Ohnishi and Hiroko Iwamura and Yoshitake Kanazawa and Tetsuya Kaneko and Shinya Kusakari and Takenori Kotani and Munemasa Mori and Yoji Murata and Hideki Okazawa and Carl F Ware and Per-Arne Oldenborg and Yoshihisa Nojima and Takashi Matozaki}, journal={Journal of immunology}, year={2011}, volume={187 1}, pages={291-7} }