Signal amplification of padlock probes by rolling circle replication.

@article{Banr1998SignalAO,
  title={Signal amplification of padlock probes by rolling circle replication.},
  author={Johan Ban{\'e}r and Mats Nilsson and Maritha Mendel-Hartvig and Ulf Landegren},
  journal={Nucleic acids research},
  year={1998},
  volume={26 22},
  pages={5073-8}
}
Circularizing oligonucleotide probes (padlock probes) have the potential to detect sets of gene sequences with high specificity and excellent selectivity for sequence variants, but sensitivity of detection has been limiting. By using a rolling circle replication (RCR) mechanism, circularized but not unreacted probes can yield a powerful signal amplification. We demonstrate here that in order for the reaction to proceed efficiently, the probes must be released from the topological link that… CONTINUE READING
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