Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide.

@article{Schmidt1997SignalAI,
  title={Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide.},
  author={Brigitte F Schmidt and J Y Chao and Zhengrong Zhu and Robbin DeBiasio and Graham Fisher},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1997},
  volume={45 3},
  pages={365-73}
}
We demonstrate that the CAtalyzed Reporter Deposition method (CARD), utilizing the novel fluorescent reporter Cy3.29-tyramide, is successful in the Fluorescent in Situ Hybridization (FISH) detection of RNA and single-copy DNA. Histone 4 expression is detected in RNA extracts of 5-phase, synchronized HeLa cells by dot-blot analysis. Gene expression of histone 4 in HeLa cells is demonstrated by FISH via CARD, utilizing oligonucleotide probes. Fluorescence intensity measurements on CARD-amplified… CONTINUE READING