Sigma-G RNA polymerase controls forespore-specific expression of the glucose dehydrogenase operon in Bacillus subtilis.

@article{Nakatani1989SigmaGRP,
  title={Sigma-G RNA polymerase controls forespore-specific expression of the glucose dehydrogenase operon in Bacillus subtilis.},
  author={Yoshihiro Nakatani and Wayne Nicholson and Katharina Neitzke and Peter Setlow and E. Freese},
  journal={Nucleic acids research},
  year={1989},
  volume={17 3},
  pages={
          999-1017
        }
}
The gene encoding glucose dehydrogenase (gdh) is part of an operon whose expression is transcriptionally activated specifically in the developing forespore of Bacillus subtilis at stage III of sporulation. The in vivo startpoint of gdh transcription was determined using primer extension analysis. Deletion mapping and site-specific mutagenesis experiments indicated that the region responsible for regulated expression of gdh in vivo was limited to the "-35" and "-10" regions preceding the… CONTINUE READING
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