Sialidase from different sources compared for electrophoretically separating serum alkaline phosphatase fractions from liver and bone.

@article{Jung1989SialidaseFD,
  title={Sialidase from different sources compared for electrophoretically separating serum alkaline phosphatase fractions from liver and bone.},
  author={Klaus Jung and M Pergande and S Klotzek},
  journal={Clinical chemistry},
  year={1989},
  volume={35 9},
  pages={1955-7}
}
We compared sialidase (neuraminidase; EC 3.2.1.18) from Vibrio cholerae, Clostridium perfringens, and Arthrobacter ureafaciens, seeking to improve the electrophoretic separation of the liver and bone isoenzymes of alkaline phosphatase (EC 3.1.3.1) on cellulose acetate membranes. Resolution is decisively determined by the type and activity of sialidase used in the preincubation of serum sample. Sialidase from Arthrobacter ureafaciens is not suited for this method. For optimal separation of the… CONTINUE READING

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