Sialic acid capture-and-release and LC-MS(n) analysis of glycopeptides.


Extracellular glycoproteins frequently carry terminal sialic acids on their N-linked and/or O-linked glycan structures. In this chapter a sialic acid specific capture-and-release protocol for the enrichment of N- and O-glycopeptides originating from glycoproteins in complex biological samples is described. The enriched glycopeptides are subjected to… (More)
DOI: 10.1007/978-1-62703-146-2_7