Shuttle expression plasmids for genetic studies in Streptococcus mutans.

  title={Shuttle expression plasmids for genetic studies in Streptococcus mutans.},
  author={I Biswas and Jyoti Krishna Jha and Nicholas Fromm},
  volume={154 Pt 8},
A set of shuttle plasmids containing four different constitutive promoters was generated to facilitate overexpression of foreign and native genes in streptococci, such as Streptococcus mutans. The four promoters that were chosen were: P(ami), P(spac), P(23) and P(veg). These promoters are active in many Gram-positive bacteria, and allow various levels of gene expression depending on the host bacterium. Shuttle plasmids were constructed based on two types of broad-host-range replication origins… CONTINUE READING
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