Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display.

@article{Swers2004ShuffledAL,
  title={Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display.},
  author={Jeffrey Seth Swers and Brenda A. Kellogg and K Dane Wittrup},
  journal={Nucleic acids research},
  year={2004},
  volume={32 3},
  pages={e36}
}
Homologous recombination in yeast can be exploited to reliably generate libraries of >10(7) transformants from a pool of PCR products and a linearized plasmid vector. Homology in the PCR insertion products drives shuffling of these genes in vivo by yeast homologous recombination. Two scFvs that share 89.8% homology were shuffled in vivo by homologous recombination, and chimeric genes were generated regardless of whether or not one of the scFv PCR products lacked 5' homology to the cut vector… CONTINUE READING

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In vitro evolution of a T cell receptor with high affinity for peptide/MHC.

Proceedings of the National Academy of Sciences of the United States of America • 2000

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