Should we be cautious on the use of commercially available antibodies to dopamine receptors?

@article{Bodei2008ShouldWB,
  title={Should we be cautious on the use of commercially available antibodies to dopamine receptors?},
  author={Serena Bodei and Nicola Arrighi and PierFranco Spano and Sandra Sigala},
  journal={Naunyn-Schmiedeberg's Archives of Pharmacology},
  year={2008},
  volume={379},
  pages={413-415}
}
Evidence indicate that it is difficult to obtain specific antibodies to G protein-coupled receptors and different technical difficulties may allow the generation of antibodies that lack specificity. We conducted experiments to validate the specificity of commercially available antibodies raised against dopamine (DA) receptors hD1, hD4, and hD5 using a transfection approach: we studied whether, in HEK 293 cells selectively transfected with the various cloned subtypes, each antibody generates… CONTINUE READING

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