Shotgun proteomics approach to characterizing the embryonic proteome of the silkworm, Bombyx mori, at labrum appearance stage.

@article{Li2009ShotgunPA,
  title={Shotgun proteomics approach to characterizing the embryonic proteome of the silkworm, Bombyx mori, at labrum appearance stage.},
  author={J Li and X Chen and S H Hosseini Moghaddam and M Chen and Hao Wei and B-X Zhong},
  journal={Insect molecular biology},
  year={2009},
  volume={18 5},
  pages={649-60}
}
The shotgun approach has gained considerable acknowledgement in recent years as a dominant strategy in proteomics. We observed a dramatic increase of specific protein spots in two-dimensional electrophoresis (2-DE) gels of the silkworm (Bombyx mori) embryo at labrum appearance, a characteristic stage during embryonic development of silkworm which is involved with temperature increase by silkworm raiser. We employed shotgun liquid chromatography tandem mass spectrometry (LC-MS/MS) technology to… CONTINUE READING