Shotgun proteomic analysis of Arabidopsis thaliana leaves.

  title={Shotgun proteomic analysis of Arabidopsis thaliana leaves.},
  author={Joohyun Lee and Wesley M. Garrett and Bret Cooper},
  journal={Journal of separation science},
  volume={30 14},
Two shotgun tandem MS proteomics approaches, multidimensional protein identification technology (MudPIT) and 1-D gel-LC-MS/MS, were used to identify Arabidopsis thaliana leaf proteins. These methods utilize different protein/peptide separation strategies. Detergents not compatible with MudPIT were used with 1-D gel-LC-MS/MS to help enrich for the detection of membrane-spanning and hydrophobic proteins. By combining the data from all MudPIT and 1-D gel-LC-MS/MS experiments, 2342 nonredundant… CONTINUE READING

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