Shotgun metagenomics, from sampling to analysis

@article{Quince2017ShotgunMF,
  title={Shotgun metagenomics, from sampling to analysis},
  author={Christopher Quince and Alan W. Walker and Jared T. Simpson and Nicholas James Loman and Nicola Segata},
  journal={Nature Biotechnology},
  year={2017},
  volume={35},
  pages={833-844}
}
Diverse microbial communities of bacteria, archaea, viruses and single-celled eukaryotes have crucial roles in the environment and in human health. However, microbes are frequently difficult to culture in the laboratory, which can confound cataloging of members and understanding of how communities function. High-throughput sequencing technologies and a suite of computational pipelines have been combined into shotgun metagenomics methods that have transformed microbiology. Still, computational… 
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