Shotgun lipidomics on a LTQ Orbitrap mass spectrometer by successive switching between acquisition polarity modes.

@article{Schuhmann2012ShotgunLO,
  title={Shotgun lipidomics on a LTQ Orbitrap mass spectrometer by successive switching between acquisition polarity modes.},
  author={Kai Schuhmann and Reinaldo R Almeida and Mark Baumert and Ronny Herzog and Stefan R. Bornstein and Andrej Shevchenko},
  journal={Journal of mass spectrometry : JMS},
  year={2012},
  volume={47 1},
  pages={96-104}
}
Top-down shotgun lipidomics relies on direct infusion of total lipid extracts into a high-resolution tandem mass spectrometer and implies that individual lipids are recognized by their accurately determined m/z. Lipid ionization efficiency and detection specificity strongly depend on the acquisition polarity, and therefore it is beneficial to analyze lipid… CONTINUE READING