Short-term primary culture of epithelial cells derived from human breast tumours.

@article{Speirs1998ShorttermPC,
  title={Short-term primary culture of epithelial cells derived from human breast tumours.},
  author={Valerie Speirs and Andrew R. Green and David S. Walton and Michael John Kerin and John N. Fox and Peter J. Carleton and Sangeeta B Desai and Stephen L Atkin},
  journal={British Journal of Cancer},
  year={1998},
  volume={78},
  pages={1421 - 1429}
}
As experimental models for breast cancer, most studies rely on established human breast cancer cell lines. However, many of these lines were established over 20 years ago, many from pleural effusions rather than the primary tumour, so the validity of using them as representative models is questionable. This paper describes our experiences, over a 3-year period, in establishing short-term epithelial-cell-enriched preparations from primary breast tumours based on differential centrifugation… CONTINUE READING