Short interfering RNA-mediated interference of gene expression and viral DNA accumulation in cultured plant cells.

@article{Vanitharani2003ShortIR,
  title={Short interfering RNA-mediated interference of gene expression and viral DNA accumulation in cultured plant cells.},
  author={Ramachandran Vanitharani and Padmanabhan Chellappan and Claude M. Fauquet},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2003},
  volume={100 16},
  pages={9632-6}
}
Gene silencing mediated by double-stranded RNA is a sequence-specific RNA degradation mechanism highly conserved in eukaryotes that serves as an antiviral defense pathway in both plants and Drosophila. Short interfering RNAs (siRNAs), the 21- to 23-nt double-stranded intermediates of this natural defense mechanism, are becoming powerful tools for reducing gene expression and countering viral infection in a variety of mammalian cells. Here we report the use of siRNAs to target reporter gene… CONTINUE READING

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