Short interfering RNA (siRNA) as a novel therapeutic.

  title={Short interfering RNA (siRNA) as a novel therapeutic.},
  author={Peter Natesan Pushparaj and Alirio J. Melendez},
  journal={Clinical and experimental pharmacology & physiology},
  volume={33 5-6},
1. RNA interference (RNAi) is a robust method of post-transcriptional silencing of genes using double-stranded RNA (dsRNA) with sequence homology driven specificity. The dsRNA can be between 21 and 23 nucleotides long: this is converted to small interfering RNA (siRNA), which then mediates gene silencing by degradation/blocking of translation of the target mRNA. 2. RNA interference provides a simple, fast and cost-effective alternative to existing gene targeting approaches both in vitro and in… CONTINUE READING